| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 281 | Thermus thermophilus | ACGACATTGAGGACGGCT | GCCACCATGCGGAAGTAGT | 58.62 | 60.08 | 274 | 52 |
100.00%
|
100.00%
|
| Primer 282 | Thermus thermophilus | TCTACGACGTGGAGGTCCA | AGCCGAACATGCGCATCA | 59.62 | 60.44 | 219 | 51 |
100.00%
|
100.00%
|
| Primer 283 | Thermus thermophilus | CCCACGCCAAGGTCATGAT | TAGGCCTCTTCACGGGTCA | 60.08 | 59.92 | 241 | 51 |
100.00%
|
100.00%
|
| Primer 284 | Thermus thermophilus | ATCCCCATCGTCACCCTCA | TGGGCAGGTTCCACTTCAC | 60.00 | 59.85 | 280 | 51 |
100.00%
|
100.00%
|
| Primer 285 | Thermus thermophilus | ATGAAGCACGTCCCCATCC | TTGGCCATCCTCACCTCCT | 59.78 | 60.23 | 252 | 51 |
100.00%
|
100.00%
|
| Primer 286 | Thermus thermophilus | TGGGACGACGAGAACCTCA | TGCACGAGCTTCCGCAAA | 60.23 | 60.59 | 170 | 51 |
100.00%
|
100.00%
|
| Primer 287 | Thermus thermophilus | TGGGACGACGAGAACCTCA | GCACGAGCTTCCGCAAAA | 60.23 | 59.36 | 169 | 51 |
100.00%
|
100.00%
|
| Primer 288 | Thermus thermophilus | ATGCCTTTGGTCCTCGCTG | AGAAGCCGTTCCGCATCA | 60.38 | 59.34 | 256 | 51 |
100.00%
|
100.00%
|
| Primer 289 | Thermus thermophilus | TGCGCTACACCGTCATCA | GCCACCTTCTGCGTGAAAG | 59.03 | 59.42 | 283 | 51 |
100.00%
|
100.00%
|
| Primer 290 | Thermus thermophilus | TGCGCTACACCGTCATCA | GCCACCTTCTGCGTGAAA | 59.03 | 58.26 | 283 | 51 |
100.00%
|
100.00%
|